Journal: bioRxiv

Article Title: Acid stress modulates metabolo-inflammatory pathways in oral epithelial cells

doi: 10.64898/2026.03.16.711383

Figure Lengend Snippet: Schematic representation of the experimental design to investigate the impact of acid stress on oral epithelial cells (OECs) and Toll-like receptor (TLR) agonist stimulation. pH Conditioning : OECs (80% confluence) were cultured in acidified (pH:=:3.0) or complete growth media (pH:=:8.0) for 24h. Morphometric Analysis ( a ): Brightfield micrographs and a machine-learning based image analysis pipeline were used to assess changes in cellular morphology. TLR Agonist Challenge : Cell cultures were subjected to either 100 ng/mL flagellin (TLR5 agonist) or 1 mg/mL Pam3CSK4 (TLR2/1 agonist) for 2-, 6-, or 24h. Molecular profiling ( b ): was conducted on OEC RNA collected after 6h TLR agonist challenge using NanoString® nCounter® technology followed by pathway analysis using the Gene Ontology knowledgebase. A TGF- β ELISA ( c ) was performed on OEC supernatants collected 2-, 6-, and 24h post TLR agonist stimulation. Figure made with Biorender.com

Article Snippet: Low-passage mixed-donor, Primary Human Oral Epithelial Cells (Cat# 36063-01, Celprogen, Inc., Torrance, USA), were thawed from liquid nitrogen, plated on poly-L-lysine-coated T-75 cell culture flasks (Sigma-Aldrich Co., St. Louis, USA; Corning Inc., Durham, USA) and maintained in Human OEC Culture Complete Growth Media (Celprogen, Inc.) containing serum and antibiotics at 37°C and 5% CO 2 for two passages prior to experimentation according to manufacturer recommendations.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Metabolites

Article Title: An NMR-Based Protocol for Profiling the Endo- and Exo-Metabolomes in Aβ 1-42 Treated Human Astrocytes from Healthy and Alzheimer’s Disease Donors

doi: 10.3390/metabo16030173

Figure Lengend Snippet: Experimental protocol. A graphical representation of the experimental protocol used for profiling of endo- and exo-metabolomes of human astrocytes by NMR. Created with BioRender.com.

Article Snippet: Authenticated human primary astrocytes (#36058-01, Celprogen Inc., Torrance, CA, USA) were obtained from six healthy donors (4 females, lot #1514431, #1514413, #1514423, #1514438, and 2 males, lot #1514449, #1514420, with a mean age of 49 ± 4.9 years, age range: 33–65 years) and from five AD patients (2 females, lot #20274, #20262, and 3 males, lot #20255, #20256, #20260, with a mean age of 83 ± 5.6 years, age range: 73–88 years).

Techniques:

Journal: Metabolites

Article Title: An NMR-Based Protocol for Profiling the Endo- and Exo-Metabolomes in Aβ 1-42 Treated Human Astrocytes from Healthy and Alzheimer’s Disease Donors

doi: 10.3390/metabo16030173

Figure Lengend Snippet: NMR-determined relative concentrations of altered metabolites in cell lysates (endo-metabolome) of astrocytes in untreated cells (C, light colours) or treated with Aβ 1-42 oligomers (Aβ, dark colours). In astrocytes from AD: ( A ) β-alanine; ( B ) creatine phosphate. In astrocytes of HS: ( C ) β-alanine; ( D ) creatine phosphate. None of these metabolites remains significant after FDR correction. Cell lysates were collected after 5 days of Aβ 1-42 oligomer treatment. * p < 0.05; ** < 0.01; ns p > 0.05. Abbreviations: C: untreated cells; Aβ: treated with Aβ 1-42 oligomers; AD: astrocytes from Alzheimer’s patients; HS: astrocytes from healthy subjects.

Article Snippet: Authenticated human primary astrocytes (#36058-01, Celprogen Inc., Torrance, CA, USA) were obtained from six healthy donors (4 females, lot #1514431, #1514413, #1514423, #1514438, and 2 males, lot #1514449, #1514420, with a mean age of 49 ± 4.9 years, age range: 33–65 years) and from five AD patients (2 females, lot #20274, #20262, and 3 males, lot #20255, #20256, #20260, with a mean age of 83 ± 5.6 years, age range: 73–88 years).

Techniques:

Journal: Metabolites

Article Title: An NMR-Based Protocol for Profiling the Endo- and Exo-Metabolomes in Aβ 1-42 Treated Human Astrocytes from Healthy and Alzheimer’s Disease Donors

doi: 10.3390/metabo16030173

Figure Lengend Snippet: NMR-determined relative concentrations of altered metabolites in astrocyte conditioned media (exo-metabolome) from untreated cells (C, light colours) or cells treated with Aβ 1-42 oligomers (Aβ, dark colours). In astrocyte media from AD: ( A ) 2-oxoglutarate; ( B ) acetate; ( C ) citrate; ( D ) glycine; ( E ) isoleucine; ( F ) leucine. In astrocyte media from C: ( G ) 2-oxoglutarate; ( H ) acetate; ( I ) citrate; ( J ) glycine; ( K ) isoleucine; ( L ) leucine. None of these metabolites remain significant after FDR correction. Conditioned media were collected after 5 days of Aβ 1-42 oligomer treatment. * p < 0.05; ** < 0.01; ns p > 0.05. Abbreviations: C: untreated cells; Aβ: treated with Aβ 1-42 oligomers; AD: astrocytes from Alzheimer’s patients; HS: astrocytes from healthy subjects.

Article Snippet: Authenticated human primary astrocytes (#36058-01, Celprogen Inc., Torrance, CA, USA) were obtained from six healthy donors (4 females, lot #1514431, #1514413, #1514423, #1514438, and 2 males, lot #1514449, #1514420, with a mean age of 49 ± 4.9 years, age range: 33–65 years) and from five AD patients (2 females, lot #20274, #20262, and 3 males, lot #20255, #20256, #20260, with a mean age of 83 ± 5.6 years, age range: 73–88 years).

Techniques: